The Nottingham born midfielder carried on for a few more minutes, before being replaced by Paul Bolland, who incidently went on to score Notts' 2nd goal in the 3-2 loss.
Brough's injury has made him doubtful for Saturday's trip to take on the Spirites at Saltergate, but there was some good news for Billy Dearden in the midfield department with the news that Darren Caskey has returned to training.
"Darren hates being in the treatment room and he hates missing training.
"I think he has probably attended more training sessions this season than any other player." added Dearden.
Paul Heffernan was withdrawn at half time last night after a clash of heads with a Wigan defender, but is expected to be fine for Saturday. The young Irishman got the knock midway through the first half and carried on until the break before Danny Allsopp replaced him and he had a bad headache following the clash of heads.