He has only recently returned to the side following a ankle ligament problem, and could be set to once more miss a match due to injury.
Brough was handed his Welsh U21 debut earlier in the season following some impressive performances, and he made a promising debut and hit the bar late on with a rasping drive.
"It was like a gunshot wound, I couldn't look at it really," Said Brough.
"I went in for a tackle and, if anything, it was me who was a little late. The lad just kept his studs in and caught me on the leg.
"Hopefully there won't be any muscle damage and I should be back soon."
He absence could be aided by the news that Paul Bolland and Richard Liburd could be back in the squad for Saturdays game with Plymouth.